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li cor odyssey blocking buffer  (LI-COR)


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    Structured Review

    LI-COR li cor odyssey blocking buffer
    Li Cor Odyssey Blocking Buffer, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 2055 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/odyssey+blocking+buffer/Intercept+(TBS)+Blocking+Buffer/pm42135436-303-4-4
    Average 98 stars, based on 2055 article reviews
    li cor odyssey blocking buffer - by Bioz Stars, 2026-09
    98/100 stars

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    Related Articles

    Membrane:

    Article Title: Mutations outside the MR1 antigen binding groove differentially inhibit presentation of exogenous antigens
    Article Snippet: Supernatants were collected after centrifugation, and whole cell lysates were combined with loading buffer and reducing agent (both Invitrogen) before loading onto a 4 to 20% polyacrylamide gel (Bio-Rad). .. Gels were run at 120V for about 1h and transferred onto a polyvinylidene fluoride membrane (Millipore), which was then blocked in Odyssey blocking buffer (Li-Cor) for at least 1h at RT. ..

    Article Title: Astrocytic Synapse Engulfment Is Differentially Controlled by APOE Genotype
    Article Snippet: .. Wash membrane in Reversal REVERT buffer for 5 mins, rocking (RT)Briefly wash membrane in PBS-T and were blocked using Odyssey blocking buffer (Licor, 927–40000) for 30 min, following overnight incubation with primary antibodies made up in Odyssey block with 0.1% Tween 20, at 4°C with gentle shaking. .. The following primary antibodies were used: Synaptophysin (mouse, Abcam ab8049, 1:1000), PSD-95 (rabbit, Cell signalling D27E11, 1:1000) and Histone (rabbit, Abcam ab1791, 1:1000).The next day, membranes were washed 6 times with PBS-0.1% Tween 20, and incubated with the following LI-COR secondary antibodies for 30 min: IRDye 680RD Donkey anti-Mouse IgG, highly cross-adsorbed (LI-COR, 925–68072, 1:5000) and IRDye 800CW Donkey anti-Rabbit IgG, highly cross-adsorbed (LI-COR, 925–32213, 1:5000).

    Article Title: Apoptotic cell clearance triggers epithelial fate reprogramming during prostate regression.
    Article Snippet: Western Blot Protein extracts were extracted from flash frozen tissue ground with mortar and pestle, using RIPA lysis buffer (10 mM Tris–HCl (pH 8.0), 140 mM NaCl, 1% Triton X-100, 0.1% sodium dodecyl sulphate, 0.1% deoxycholate, 1 mM EDTA) supplemented with protease and phosphatase inhibitor cocktails (Roche 11697498001). .. After being run for 2 hours at 100V on a 12% resolving polyacrylamide gel, proteins (20ug/sample) were transferred to an Immobilon FL PVDF membrane (Millipore IPFL00010) and probed with the following antibodies diluted in Odyssey blocking buffer (LI-COR): anti-PanKLa, and anti-Histone H3. .. Rabbit IR dye secondary antibodies (LI-COR Biosciences) were used at 1:20 000.

    Blocking Assay:

    Article Title: Mutations outside the MR1 antigen binding groove differentially inhibit presentation of exogenous antigens
    Article Snippet: Supernatants were collected after centrifugation, and whole cell lysates were combined with loading buffer and reducing agent (both Invitrogen) before loading onto a 4 to 20% polyacrylamide gel (Bio-Rad). .. Gels were run at 120V for about 1h and transferred onto a polyvinylidene fluoride membrane (Millipore), which was then blocked in Odyssey blocking buffer (Li-Cor) for at least 1h at RT. ..

    Article Title: Astrocytic Synapse Engulfment Is Differentially Controlled by APOE Genotype
    Article Snippet: .. Wash membrane in Reversal REVERT buffer for 5 mins, rocking (RT)Briefly wash membrane in PBS-T and were blocked using Odyssey blocking buffer (Licor, 927–40000) for 30 min, following overnight incubation with primary antibodies made up in Odyssey block with 0.1% Tween 20, at 4°C with gentle shaking. .. The following primary antibodies were used: Synaptophysin (mouse, Abcam ab8049, 1:1000), PSD-95 (rabbit, Cell signalling D27E11, 1:1000) and Histone (rabbit, Abcam ab1791, 1:1000).The next day, membranes were washed 6 times with PBS-0.1% Tween 20, and incubated with the following LI-COR secondary antibodies for 30 min: IRDye 680RD Donkey anti-Mouse IgG, highly cross-adsorbed (LI-COR, 925–68072, 1:5000) and IRDye 800CW Donkey anti-Rabbit IgG, highly cross-adsorbed (LI-COR, 925–32213, 1:5000).

    Article Title: Beta cell-derived cholecystokinin drives obesity-associated pancreatic adenocarcinoma development.
    Article Snippet: .. Membranes were blocked using Odyssey Blocking Buffer (Licor) in PBS (1:2) for one hour, then incubated with primary antibodies diluted in Odyssey Blocking Buffer and PBS-T (1:2) overnight at 4 °C. .. Membranes werewashed for 10minuteswith PBS-T three times and then incubated in DyLight secondary antibodies (Cell Signaling Technologies) for one hour at room temperature.

    Article Title: Apoptotic cell clearance triggers epithelial fate reprogramming during prostate regression.
    Article Snippet: Western Blot Protein extracts were extracted from flash frozen tissue ground with mortar and pestle, using RIPA lysis buffer (10 mM Tris–HCl (pH 8.0), 140 mM NaCl, 1% Triton X-100, 0.1% sodium dodecyl sulphate, 0.1% deoxycholate, 1 mM EDTA) supplemented with protease and phosphatase inhibitor cocktails (Roche 11697498001). .. After being run for 2 hours at 100V on a 12% resolving polyacrylamide gel, proteins (20ug/sample) were transferred to an Immobilon FL PVDF membrane (Millipore IPFL00010) and probed with the following antibodies diluted in Odyssey blocking buffer (LI-COR): anti-PanKLa, and anti-Histone H3. .. Rabbit IR dye secondary antibodies (LI-COR Biosciences) were used at 1:20 000.

    Article Title: Interplay of SLC33A1-dependent and -independent Golgi sialic acid O -acetylation in CASD1 catalysis
    Article Snippet: .. After blotting, PVDF membranes were blocked with Odyssey blocking buffer (LI-COR Biosciences) diluted 1:2 with PBS. .. Antibody staining was performed in the same buffer system using mouse anti-V5 mAb (0.1 μg mL −1 ; Acris cat. SM1691PS, clone SV5-PK1) followed by goat anti-mouse IgG IRDye 800CW-conjugate (1:20,000; LI-COR Biosciences cat. 926-32210) and rabbit anti-Actin pAb (1:1000; Sigma-Aldrich cat. A2066) followed by anti-rabbit IgG IRDye 800CW-conjugate (1:20,000; LI-COR Biosciences cat. 926-32211).

    Article Title: Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles.
    Article Snippet: .. Following electrophoresis, proteins were transferred onto PVDF membranes, which were then blocked using Odyssey blocking buffer (LI-COR Biosciences) or 5% non-fat milk in TBS-0.1%T to minimize non-specific binding. .. The primary antibodies for Western blots used in this study were EFEMP1 (1:300, Novus Biologicals, NBP1-77040), β-Actin (1:2000, Cell Signaling Technology, 4970 or 3700S), STAT1 (1:1000, Cell Signaling Technology, 9172S), CD63 (1:1000, Novus Biologicals, NBP2-42225), CD81 (1:1000, Novus Biologicals, NB100-65805SS), vinculin (1:10,000, Sigma-Aldrich, V9131200UL) and Calnexin (1:1000, Novus Biologicals, NB100-1965SS).

    Article Title: Environmental pollutant BDE-47 alters glycan and microRNA signatures of macrophage-derived extracellular vesicles and modulates senescence signaling.
    Article Snippet: .. After transfer blocking was performed using Odyssey® Blocking Buffer (OBB, LI-COR) diluted in TBS, followed by incubation with primary antibodies anti-β-actin (SigmaAldrich), anti-p21 (Cell Signaling Technologies), anti-p53 and anti-p16 (Santa Cruz Biotechnology) diluted in OBB. .. Detection was carried out using IRDye® 800CW or Alexa Fluor 680-conjugated secondary antibodies (LI-COR; Invitrogen), and membranes were scanned with the Odyssey IR scanner (LICOR Biosciences, Lincoln, NE, USA).

    Incubation:

    Article Title: Astrocytic Synapse Engulfment Is Differentially Controlled by APOE Genotype
    Article Snippet: .. Wash membrane in Reversal REVERT buffer for 5 mins, rocking (RT)Briefly wash membrane in PBS-T and were blocked using Odyssey blocking buffer (Licor, 927–40000) for 30 min, following overnight incubation with primary antibodies made up in Odyssey block with 0.1% Tween 20, at 4°C with gentle shaking. .. The following primary antibodies were used: Synaptophysin (mouse, Abcam ab8049, 1:1000), PSD-95 (rabbit, Cell signalling D27E11, 1:1000) and Histone (rabbit, Abcam ab1791, 1:1000).The next day, membranes were washed 6 times with PBS-0.1% Tween 20, and incubated with the following LI-COR secondary antibodies for 30 min: IRDye 680RD Donkey anti-Mouse IgG, highly cross-adsorbed (LI-COR, 925–68072, 1:5000) and IRDye 800CW Donkey anti-Rabbit IgG, highly cross-adsorbed (LI-COR, 925–32213, 1:5000).

    Article Title: Beta cell-derived cholecystokinin drives obesity-associated pancreatic adenocarcinoma development.
    Article Snippet: .. Membranes were blocked using Odyssey Blocking Buffer (Licor) in PBS (1:2) for one hour, then incubated with primary antibodies diluted in Odyssey Blocking Buffer and PBS-T (1:2) overnight at 4 °C. .. Membranes werewashed for 10minuteswith PBS-T three times and then incubated in DyLight secondary antibodies (Cell Signaling Technologies) for one hour at room temperature.

    Article Title: Environmental pollutant BDE-47 alters glycan and microRNA signatures of macrophage-derived extracellular vesicles and modulates senescence signaling.
    Article Snippet: .. After transfer blocking was performed using Odyssey® Blocking Buffer (OBB, LI-COR) diluted in TBS, followed by incubation with primary antibodies anti-β-actin (SigmaAldrich), anti-p21 (Cell Signaling Technologies), anti-p53 and anti-p16 (Santa Cruz Biotechnology) diluted in OBB. .. Detection was carried out using IRDye® 800CW or Alexa Fluor 680-conjugated secondary antibodies (LI-COR; Invitrogen), and membranes were scanned with the Odyssey IR scanner (LICOR Biosciences, Lincoln, NE, USA).

    Gentle:

    Article Title: Astrocytic Synapse Engulfment Is Differentially Controlled by APOE Genotype
    Article Snippet: .. Wash membrane in Reversal REVERT buffer for 5 mins, rocking (RT)Briefly wash membrane in PBS-T and were blocked using Odyssey blocking buffer (Licor, 927–40000) for 30 min, following overnight incubation with primary antibodies made up in Odyssey block with 0.1% Tween 20, at 4°C with gentle shaking. .. The following primary antibodies were used: Synaptophysin (mouse, Abcam ab8049, 1:1000), PSD-95 (rabbit, Cell signalling D27E11, 1:1000) and Histone (rabbit, Abcam ab1791, 1:1000).The next day, membranes were washed 6 times with PBS-0.1% Tween 20, and incubated with the following LI-COR secondary antibodies for 30 min: IRDye 680RD Donkey anti-Mouse IgG, highly cross-adsorbed (LI-COR, 925–68072, 1:5000) and IRDye 800CW Donkey anti-Rabbit IgG, highly cross-adsorbed (LI-COR, 925–32213, 1:5000).

    Electrophoresis:

    Article Title: Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles.
    Article Snippet: .. Following electrophoresis, proteins were transferred onto PVDF membranes, which were then blocked using Odyssey blocking buffer (LI-COR Biosciences) or 5% non-fat milk in TBS-0.1%T to minimize non-specific binding. .. The primary antibodies for Western blots used in this study were EFEMP1 (1:300, Novus Biologicals, NBP1-77040), β-Actin (1:2000, Cell Signaling Technology, 4970 or 3700S), STAT1 (1:1000, Cell Signaling Technology, 9172S), CD63 (1:1000, Novus Biologicals, NBP2-42225), CD81 (1:1000, Novus Biologicals, NB100-65805SS), vinculin (1:10,000, Sigma-Aldrich, V9131200UL) and Calnexin (1:1000, Novus Biologicals, NB100-1965SS).

    Binding Assay:

    Article Title: Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles.
    Article Snippet: .. Following electrophoresis, proteins were transferred onto PVDF membranes, which were then blocked using Odyssey blocking buffer (LI-COR Biosciences) or 5% non-fat milk in TBS-0.1%T to minimize non-specific binding. .. The primary antibodies for Western blots used in this study were EFEMP1 (1:300, Novus Biologicals, NBP1-77040), β-Actin (1:2000, Cell Signaling Technology, 4970 or 3700S), STAT1 (1:1000, Cell Signaling Technology, 9172S), CD63 (1:1000, Novus Biologicals, NBP2-42225), CD81 (1:1000, Novus Biologicals, NB100-65805SS), vinculin (1:10,000, Sigma-Aldrich, V9131200UL) and Calnexin (1:1000, Novus Biologicals, NB100-1965SS).



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